CH60033
Principle
Zilpaterol rapid test kit is based on lateral flow immunochromatographic assay. The test
device has a testing window. The testing window has an invisible T (test) zone and C
(control) zone. When sample is applied into the sample hole on the device, the liquid
will laterally flow on the surface of the test strip. If there is little Zilpaterol in the sample,
a visible T band will appear; T band is invisible if it has more Zilpaterol in the sample. The
C band should always appear after a sample is applied, indicating a valid result. By this
means, the device can accurately indicate the presence of Zilpaterol in the feed, urine,
tissue sample.
Sample Preparation
Tissue, urine, feed
Technical Specification
Detect limit: 10μg/kg(ppb)
Reaction temperature: 25℃
Detect time: 10min.
Sample Preparation
-Tissue
1. Homogenize the sample.
Take 5±0.05g in 10mL centrifuge tube, hot in water batch for 10min.
2. Return to room temperature.
3. Take 100µL for analysis.
-Urine
1. Take 5mL urine smaple,centrifuge at 4000 rpm for 5 min at room temperature.
2. Take 100µL clear layer for analysis.
-Feed
1. Take 1g grinded sample, add 3mL deionized water, shake for 10min with oscillator .
2. Centrifuge at 4000 rpm for 5min at room temperature(or Filter liquid sample using
Whatman No.1).
3. Take 100µL lower layer for analysis
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