Capsaicin Assay ELISA Test Kit for Pepper Oil and Hotpot Condiment Analysis
Product Code: CH97057
Product Overview
This competitive enzyme immunoassay test kit provides quantitative analysis of capsaicin content to measure the spiciness of peppers and pepper-based products. The kit supports up to 96 total tests, with capacity for 47 samples when conducting duplicate testing.
Key Features
- Rapid processing with results in under 10 minutes
- High recovery rate of ≥80% with efficient pre-processing methods
- Detection limit: 1 degree of spiciness
- Quantitative limit: 2 degrees of spiciness
- Fast detection time of 30 minutes
- Excellent reproducibility across tests
Sample Preparation Instructions
Sample Extraction Solution Preparation
Mix 70mL methanol with 30mL dimethyl sulfoxide and set aside for use.
Pepper Oil Sample Preparation
(1) Weigh 0.5g homogenized sample into container, add 25mL sample extraction solution, vortex for 2 minutes
(2) Centrifuge at 4000rpm for 5 minutes
(3) Take 0.1mL supernatant, add 9.9mL deionized water, mix well
(4) Take 0.1mL mixed solution, add 0.9mL deionized water, mix well
(5) Take 0.1mL mixed solution, add 0.1mL sample diluent, mix well
Dilution Factor: 1
For samples exceeding the detection limit: Add an additional dilution step between steps (2) and (3) by diluting the liquid from step (2) with methanol to an appropriate multiple before proceeding. Multiply the final result by this dilution multiple.
Hotpot Condiment Sample Preparation
Hot pot base should be heated until melted before weighing. Samples containing solid particles require homogenization first.
(1) Weigh 3.0g homogenized sample into container, add 30mL sample extraction solution, vortex for 2 minutes
(2) Centrifuge at 4000rpm for 5 minutes
(3) Take 1mL supernatant, add 4mL sample extraction solution, mix well
(4) Take 0.1mL mixed liquid, add 9.9mL deionized water, mix well
(5) Take 0.1mL mixed solution, add 0.9mL deionized water, mix well
(6) Take 0.1mL mixed solution, add 0.1mL sample diluent, mix well
Dilution Factor: 1
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